Abstract
1. 1. The concentration of ferritin in liver from male and female rats at different ages has been determined by a single radial immunodiffusion technique. Low levels of ferritin iron and ferritin protein were found in both sexes from a week after weaning to about 7 weeks of age. From then on the levels increased, and the increase was about twice as rapid in livers from female rats compared to those from male rats. Ferritin from female rats contained slightly more iron than ferritin from males, and the degree of iron saturation increased as the rats of both sexes developed. The identity of apoferritins from male and female liver has been verified by double immunodiffusion and by polyacrylamide gel disc electrophoresis. 2. 2. A new method for small scale purification of ferritin, based on polyacrylamide gel disc electrophoresis, is described. The purity of the isolated ferritin has been verified by disc electrophoresis at different pH and gel concentrations and by immuno-electrophoresis. 3. 3. Incorporation of [ 14C]leucine into ferritin of isolated perfused male rat liver seemed to be stimulated by preadministration of estradiol-17-β in vivo.
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