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Abstract
Sevoflurane is widely used for anesthesia, and is commonly used together with opioids in clinical practice. However, the effects of sevoflurane on µ-opioid receptor (µOR) functions is still unclear. In this study, the effects of sevoflurane on µOR functions were analyzed by using Xenopus oocytes expressing a µOR fused to chimeric Gα protein G<sub>qi5</sub> (µOR-G<sub>qi5</sub>). Sevoflurane by itself did not elicit any currents in oocytes expressing µOR-G<sub>qi5</sub>, whereas sevoflurane inhibited the [D-Ala<sup>2</sup>,N-Me-Phe<sup>4</sup>,Gly<sup>5</sup>-ol]-enkephalin (DAMGO)-induced Cl<sup>–</sup> currents at clinically used concentrations. Sevoflurane did not affect the Cl<sup>–</sup> currents induced by AlF<sub>4</sub><sup>–</sup>, which directly led to activation of G proteins. The inhibitory effects of sevoflurane on the DAMGO-induced currents were not observed in oocytes pretreated with the protein kinase C (PKC) inhibitor GF109203X. These findings suggest that sevoflurane would inhibit µOR function. Further, the mechanism of inhibition by sevoflurane would be mediated by PKC.
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