Abstract
Purpose: To investigate the protective effect of sevoflurane against lipopolysaccharide (LPS)-induced acute liver injury (ALI) in amice model.Methods: Seven week-old female BalB/C mice were used. Lung water content and cell count were estimated by standard protocols. Cytokine and chemokine analysis was performed using commercially available kits. Myeloperoxidase activity was evaluated spectrophotometrically while histopathological analysis was carried out by H and E staining.Results: The results revealed that sevoflurane treatment significantly improved gaseous exchange, and reduced lung water content and lung inflammation as evidenced by a decrease in neutrophil migration into BALF (p < 0.01). Sevoflurane also significantly reversed the LPS-triggered suppression of IL-10 in the lung tissues of LPS-treated mice, when compared to saline-treated controls (p < 0.01). It reversed LPS-induced oxidative stress, as demonstrated by increase in total antioxidant capacity (T-AC), catalase (CAT) and superoxide dismutase-1 (SOD-1), as well as an increase in reduced/oxidized glutathione (GSH/GSSG) ratio. In addition, sevoflurane blocked LPS-induced lung tissue injury in ALI mice, and exerted protective effects against acute LPS-induced lung injury.Conclusion: These results suggest that sevoflurane improves gaseous exchange and exerts a protective effect against LPS-triggered lung injury in mice model, most probably due to its antiinflammatory and antioxidant properties.Keywords: Lung injury, Sevoflurane, Respiratory distress, Superoxide dismutase, Liposaccharide
Highlights
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS1) cases are seen frequently in intensive care units (ICUs) [1]
The effect of sevoflurane on gaseous exchange, antioxidant defense system and LPS-induced lung tissue injuries was investigated in a mice model of ALI
Lung inflammation in relation to neutrophil counts triggered by LPS was assessed by determining cell profile of broncho-alveolar lavage fluid (BALF) 24 h succeeding intra-nasal instillation
Summary
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS1) cases are seen frequently in intensive care units (ICUs) [1]. The effect of sevoflurane on gaseous exchange, antioxidant defense system and LPS-induced lung tissue injuries was investigated in a mice model of ALI. To determine lung water content, the lung tissues were first weighed and dried in line with the protocol described previously [14]. Myeloperoxidase (MPO) activity was determined to evaluate infiltration of neutrophils in the lung tissues of LPS-induced ALI mouse [15,16,17]. Rise in water contents of the lungs was observed in group III animals (LPS-treated), relative to group I (saline-treated), and group II mice treated with sevoflurane only. The water contents in the lungs of group IV (LPS + sevoflurane) and group V (LPS + dexamathasone) were more or less similar, but significantly higher than that of group II animals (p < 0.01). The results were considered significant at p < 0.05 and p < 0.01
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