Severity of Tomato brown rugose fruit virus in tomato (Solanum lycopersicum L.) from a region of Coahuila, México

Abstract

The purpose of this research was to describe the Tomato brown rugose fruit virus, from three isolates collected in the field and also to use a diagrammatic scale of severity for its evaluation. The isolation was carried out with the collection of 200 ha-1 leaflets, according to a statistical method, from commercial greenhouses. Agdia® immunological strips were used to confirm the symptoms and the virus was identified by RT-PCR. A completely randomized experimental design was established in tomato plants var. Río Grande®, with three treatments or isolates and five repetitions: Blindom F1® tissue, Quiroga® Enza zaiden tissue and Quiroga® Enza zaiden fruit; Controls with phosphate buffer and buffer+celite were used as negative control. The trial began with the inoculation of 45-day-old tomato plants, where only the primary leaves were inoculated. Fertilization was carried out twice a week using macro and microelements from commercial companies. To quantify the percentage of damage to foliage and fruit, a diagrammatic scale of severity was used. The three isolates evaluated differed in the symptoms produced by ToBRFV, where; The Fruto Quiroga® Enza zaiden isolate stood out, with a higher incidence, severity and shorter incubation period compared to the two isolates evaluated.