Severe incomplete fusion of the Müllerian ducts influences reproduction in Holstein cattle

Abstract

Fusion failure of the Müllerian ducts is thought to occur congenitally in cattle. We aimed to elucidate the contribution of incomplete fusion of the Müllerian ducts to reproductive difficulties in dairy cattle. We observed the vaginas of Holstein cattle to classify the anomalies into mild and severe types, based on severity of incomplete fusion, and recorded information about the cattle at the time of artificial insemination (AI) or embryo transfer. Of the 1054 Holstein cattle examined, 22 (2.09%) individuals showed incomplete fusion of the Müllerian ducts. Among them, 17 (77.3%) had mild type and 5 (22.7%) had severe type incomplete fusion. We analyzed the changes in the prevalence of these anomalies in previous studies and the present study. The prevalence of incomplete fusion of the Müllerian ducts varied from 0% to 6.98% by dairy breed or region. Linear regression analysis showed that the change in the prevalence over time was not statistically significant, with a regression coefficient of −0.04% per year (r2 = 0.27; P = 0.07). The effect of incomplete fusion of the Müllerian ducts on reproductive performance was evaluated by univariate analysis: first service pregnancy rate, number of services, and days from first service to pregnancy were significantly affected in the heifers with the severe types. We next analyzed the effect of incomplete fusion of the Müllerian ducts on conception, using logistic regression analysis. Mild and severe types of incomplete fusion of the Müllerian ducts were selected as explanatory variables, along with heat stress, parity, the number of previous services, AI after ovulation, and sex-sorted semen. The severe types (OR = 0.24, P = 0.03), but not the mild types (OR = 1.01, P = 0.98), were significantly associated with conception. In the present study, we divided the incomplete fusion of the Müllerian ducts by severity and demonstrated that the severe types had a significant effect on poor conception in Holstein cattle. Since the adjusted odds of conception of the severe types of incomplete fusion of the Müllerian ducts were approximately 4 times lower than those of the normal cattle, it is important to determine severe incomplete fusion of the Müllerian ducts prior to service.