Abstract
The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI). After baseline MRI, 24 Spraque-Dawley rats with renal IRI were divided equally as group 1 (post-IRI MRI at 6 hours, days 1, 3, and 7) and groups 2, 3, and 4 (post-IRI MRI at 6 hours; 6 hours and day 1; 6 hours, days 1 and 3, respectively), while six other rats without IRI (group 5) were used as sham control. ADC, T1, and T2 values of the cortex and outer and inner stripes of outer medulla (OSOM and ISOM), and immunohistochemical studies assessing monocyte chemoattractant protein-1 (MCP-1), CD68+ cells, tubular cast formation, and collagen deposition in three zones at different time points were evaluated. Significantly reduced ADCs in OSOM and ISOM are noninvasive biomarkers denoting hyperacute damages after IRI. Linear regression analysis revealed a significant inverse correlation between 6-hour/baseline ADC ratios and MCP-1 staining (P < 0.001, r2 = 0.738). ADC, T1, and T2 values are useful for assessing variable IRI changes in different layers depending on underlying microstructural and histopathological changes at different time points.
Highlights
The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI)
Consistent with prior studies, even after severe IRI of both kidneys, our results revealed that the serum creatinine changes were less apparent initially for several hours, while subsequent rapid deterioration of renal function occurred in hours and such a lag time between renal injury and the increase in the serum creatinine level can lead to missed early therapeutic opportunities[6, 24]
We found that the baseline ADChigh value of the cortex was significantly lower than the baseline ADCavg while the OSOM and inner stripe of outer medulla (ISOM) showed non-significant decrement, indicating affluent cortical perfusion can affect true diffusion measurement
Summary
The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI). Despite the various risk factors of AKI that have been reported, renal tissue hypoxia or ischemic-reperfusion injury (IRI) is closely related to the pathophysiology of various initiating insults[3, 6,7,8]. Blood oxygen level-dependent imaging for measuring renal parenchymal hypoxia and diffusion-weighted imaging for assessing restricted Brownian diffusion with decreased apparent diffusion coefficient (ADC) in fibrotic AKI kidney have been reported[10, 13]. The purpose of the present study was to investigate the hyperacute and acute changes in ADC, T1, and T2 mapping after severe bilateral renal IRI with longitudinal and cross-sectional assessments, and immunohistochemical (IHC) correlations in a rat model
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
