Severe asynapsis in spermatocytes of interspecific hybrids of the silver fox (Vulpes vulpes) and the blue fox (Alopex lagopus) leads to pachytene I arrest as a result of sustained H2AXγ phosphorylation

Abstract

Infertility is frequently associated with meiotic anomalies which can result in the production of chromosomally abnormal gametes or be concomitant with meiotic arrest. We investigated whether spermatocytes of male interspecific hybrids of the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus) presented alterations in chromosomal synapses and meiotic checkpoint signalling. Using the immunofluorescence technique with SP1 and SP3 proteins, bivalent structures and their deviations (multivalents, univalents and not fully conjugated bivalents) were analyzed on meiotic preparations. This technique allowed the localization of frequent foci of phosphorylated histones H2AHγ (Ser 139) to the meiotic block in late pachytene. These results indicate a disruption of meiotic division in male fox hybrids, which leads to a high percentage of apoptotic cells in the gonads of these animals and, consequently, sterility.