Abstract
BackgroundIn recent years, an increasing number of studies have proved that circulating miRNAs could be used for the early diagnosis of cardiovascular diseases and even play vital roles in the evaluation of therapeutic effects or prognosis. This study was conducted to examine the correlation between serum microRNAs and hyperlipidemia to provide a theoretical basis for the early screening and intervention of atherosclerotic cardiovascular diseases (ASCVD).MethodsThe serum samples and clinical data of 122 patients with hyperlipidemia and 168 healthy subjects were collected. Related clinical information was statistically analyzed for the two groups. Expression of circulating miRNAs was detected by miRNA microarray analysis and further verified by reverse transcription-quantitative PCR (RT-qPCR).ResultsStatistical analysis of clinical information revealed a significant difference in the incidence of ASCVD between the two groups. The MiRNA microarray analysis (n = 10) showed 22 miRNAs with significantly different expression, among which 12 showed upregulation, and the others showed downregulation. Those possessing obvious differences and stable expression in the miRNA microarray, including miRNA-191-3p, miRNA-933, and miRNA-425-3p, were chosen for further investigation using RT-qPCR. The results demonstrated that several miRNAs were related to lipid metabolism disorders, especially miRNA-933. The area under the curve (AUC) of miRNA-933 in distinguishing the hyperlipidemia and ASCVD patients was 0.739 (95% CI, 0.682–0.795; P < 0.01) and 0.703 (95% CI, 0.643–0.763, P < 0.01), respectively.ConclusionsIn conclusion, miRNA-191-3p, miRNA-933, and miRNA-425-3p may be depressed in the peripheral circulation of patients with lipid metabolism disorders (mainly LDL). Circulating miRNA-933 could be a feasible predictor for ASCVD at the early stage.
Highlights
Atherosclerotic cardiovascular disease (ASCVD) is defined as a group of clinical diseases mostly caused by endothelial dysfunction based on atherosclerosis (AS), including coronary heart disease, stroke, and other various cardiovascular diseases [1]
Additional studies revealed the dynamic alterations of circulating miRNA expression could be detected under different pathological conditions, including various cardiovascular diseases, thereby indicating that detecting the different expression of miRNAs in peripheral blood circulation may help in the early diagnosis of atherosclerotic cardiovascular diseases [10, 11]
Our dates revealed a significant difference in the incidence of both carotid atherosclerosis and atherosclerotic cardiovascular diseases (ASCVD) between the two groups
Summary
Atherosclerotic cardiovascular disease (ASCVD) is defined as a group of clinical diseases mostly caused by endothelial dysfunction based on atherosclerosis (AS), including coronary heart disease, stroke, and other various cardiovascular diseases [1]. MiRNAs could regulate gene expression on the posttranscriptional level by binding to the 3’UTR of mRNA, which may either inhibit the translation of protein from mRNA or promote the degradation of mRNA. These RNAs participate in most physiological and pathological processes in organisms [6]. This study was conducted to examine the correlation between serum microRNAs and hyperlipidemia to provide a theoretical basis for the early screening and intervention of atherosclerotic cardiovascular diseases (ASCVD)
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