Abstract
Detected are several differences in biochemical properties of V. cholerae 569 B Inaba and V. cholerae M41 Ogawa productive strains. The differences are in the protein content in culture liquid, in the dynamics of enzyme activity in the process of reactor strain growth, and in the protease, phospholipase A2 and C activity. Demonstrated is the diverse resistance of enzymes of both of the strains to formaldehyde detoxification effect. Identified is the difference in enzyme activity of fractions, which are obtained by means of isoelectric precipitation of culture liquid proteins within the limits of pH 3,5-4,4.
Highlights
Detected are several differences in biochemical properties of V. cholerae 569 B Inaba and V. cholerae M41 Ogawa productive strains
The differences are in the protein content in culture liquid, in the dynamics of enzyme activity in the process of reactor strain growth, and in the protease, phospholipase A2 and C activity
Identified is the difference in enzyme activity of fractions, which are obtained by means of isoelectric precipitation of culture liquid proteins within the limits of pH 3,5–4,4
Summary
Detected are several differences in biochemical properties of V. cholerae 569 B Inaba and V. cholerae M41 Ogawa productive strains. Целью настоящей работы явилось сопоставление активности ряда ферментов, присутствующих в культуральной жидкости у штаммов 569В и М41 холерного вибриона, что позволит более полно охарактеризовать материал, идущий на изготовление вакцины. В опытах по изоэлектрическому осаждению белков культуральной жидкости полученные осадки, отделенные центрифугированием и растворенные в 0,05 М трис-буфере (рН 8,0), и надосадочную жидкость, сконцентрированную в 10 раз и нейтрализованную до рН 7,0, использовали для определения активности ферментов и белка.
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