Abstract
THERE are numerous methods for estimating concentrations of spermatozoa. Rothschild (1950) lists six of them: haemocytometer counts, estimations of total nitrogen, weights of dried semen, visual comparisons with standard opacity tubes, electrical resistances of sperm suspensions, and absorptiometric measurements of opacity.Any method for estimating the number of spermatozoa in a sample of semen must be standardized by actual count. This is usually done on the haemocytometer in a way similar to that used for the enumeration of red blood corpuscles (Todd and Sanford, 1939).After filling a haemocytometer with diluted semen, the first observation reveals that all spermatozoa are not in the same plane of focus under the microscope. If the lines of the haemocytometer are in focus, the number of sperm observed changes with time, that is, the spermatozoa settle. Counting the spermatozoa can begin while they are settling, but it is necessary to change focus to see .
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