SETTING OF PARAMETERS OF ACUTE AND SUBACUTE TOXICITY OF THE POWDER BASED ON IODOFORM

Abstract

The article presents the results of establishing the parameters of acute and subacute toxicity of the powder based on iodoform.
 Determination of acute toxicity parameters of the drug "Iodomin" were performed on 30 white mice 2-3 months of age, weighing 20-23 g and 30 white rats, aged 2-3 months, weighing 170-190 g. The drug was administered intragastrically, once, pre-dissolved in water. As a result of studies to determine acute toxicity by oral administration, it was found that, after administration of the drug in doses of 1000, 3000 and 5000 mg/kg, all animals remained alive. No changes in the clinical condition of the animals of the experimental groups were observed. The DL50 of ''Iodomin" powder is more than 5000 mg/kg. In conducted studies did not reveal the death of animals, respectively, the tested drug belongs to the IV class of toxicity (low toxicity).
 Studies on the determination of acute dermal toxicity of Iodomine were performed in accordance with the requirements of OECD № 402 (Acute Dermal Toxicity: Fixed Dose Procedure, 2017). Studies have shown that skin application of powder "Iodomin" at a dose of 2000 mg/kg body weight did not cause death, appearance of toxic effects. According to GHS the drug belongs to the 5th category.
 In the study of subacute toxicity, the drug was administered intragastrically, daily, pre-dissolved in water. On the 4th day of the experiment, in two experimental groups was a significant decrease in heart weight, which may be associated with functional load, and in group 2 (5-fold from therapeutic dose), along with that was a significant increase in liver mass, decreased concentration hemoglobin in the erythrocyte, creatinine in the blood, increased ALT activity.
 To determine the harmful effects of Iodomin powder on the mucous membrane of the eye were used 3 rabbits, to which the drug was administered in the amount of 2 drops in the conjunctival sac of the left eye. The harmful effect of the tested substance on the mucous membrane of the eyes was assessed by the appearance of hyperemia, edema and secretions according to the scoring system. When applying the suspension on the mucous membrane of the eye, it was found out, that after 24-48 hours the drug does not cause irritation. Also was found that the studied agent does not cause hyperemia, edema and changes in blood vessels. It's established that the powder "Iodomin" does not cause harmful effects on the mucous membranes of the eye.