Abstract
<p><strong>Objective: </strong>To investigate the neuroprotective effect of sesamol against rotenone-induced cell death in SH-SY5Y cells associated with Parkinsonism.</p><p><strong>Methods: </strong>SH-SY5Y cells were maintained in Dulbecco’s modified Eagle’s medium. After differentiation, the cells were incubated with rotenone (20 μM) and sesamol at different concentrations (10-100 μM). Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. The reactive oxygen species, mitochondrial membrane potential and nuclear morphology were determined by dichlorofluorescein diacetate, rhodamine 123 and 4’, 6-diamidino-2-phenylindole, respectively. Thiobarbituric acid reactive substances, activities of catalase, superoxide dismutase, and glutathione peroxidase and glutathione level were determined by standard assays.</p><p><strong>Results: </strong>Sesamol significantly increased the cell viability and decreased the rotenone-induced cell death in SH-SY5Y cells. Sesamol antagonized rotenone-induced reactive oxygen species generation, loss of mitochondrial membrane potential and nuclear damage. Sesamol also decreased thiobarbituric acid reactive substances level, increased the activities of catalase, superoxide dismutase, glutathione peroxidase and increased the level of glutathione in rotenone-induced cells.</p><p><strong>Conclusion: </strong>The results obtained strongly indicate the promising neuroprotective role of sesamol against rotenone-induced death in SH-SY5Y cells.</p>
Highlights
Parkinson’s disease (PD) is an overwhelming neurodegenerative movement disorder in industrialized countries
Sesamol, retinoic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl-tetrazolium bromide (MTT), dichlorofluorescein diacetate (DCFH-DA), rhodamine 123, 4’,6-diamidino-2-phenylindole (DAPI), dimethyl sulphoxide (DMSO), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and other tissue culture reagents were purchased from Sigma-Aldrich
We evaluated the neuroprotective effect of SES on ROTinduced SH-SY5Y cells
Summary
Parkinson’s disease (PD) is an overwhelming neurodegenerative movement disorder in industrialized countries. Several toxicant-induced model systems have been developed to study Parkinson’s disease including rotenone (ROT), MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), 6-hydroxydopamine (6-OHDA) and paraquat [2]. The increased risk of PD is associated with exposure to organic pesticide such as ROT [3] These findings have renewed interest in the link between exposure to pesticides and the development of Parkinson’s disease [4]. Et al [10] reported that natural free radical scavenger could provide antioxidant mechanisms to nullify the negative effects in cells. Khadira Screen and Vijayalakshmi have reported SES as an efficient antioxidant [12] which protects the cells from free radical injury. The rationale of the study is to investigate the neuroprotective role of SES in ROTinduced SH-SY5Y cells
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