Sesame Genome Assembly

Abstract

AbstractGenome assembly strategy is a crucial step for reconstructing high-quality genomes in any genome project. With the continuous innovation of new sequencing platforms, various methods of genome assembly have been developed and applied in genome assembly. In order to construct a fine genome map for sesame, the Sesame Genome Working Group (SGWG) group analyzed and optimized the assembly strategy for sesame according to the various sequencing data and genome characteristics. The comprehensive genome assembly strategy for sesame comprised the next generation sequencing (NGS), the third assembly platforms, and Hi-C assembly strategy with supplementation of high-dense single nucleotide polymorphism (SNP) genetic map and bacterial artificial chromosome—fluorescent in situ hybridization (BAC-FISH) cytogenetic map. Comparison results of the specific and high-efficient assembly techniques in sesame were discussed in this chapter. The final assembled genome of sesame (cv. Yuzhi 11) comprises of 335 megabase (Mb) sequences with high completeness. About 313 Mb sequences are assembled into the 13 chromosome molecules. High accuracy and integrity of the assembled genome indicates the suitable genome assembly for sesame. The results provide an extraordinary example of the application of high-quality genome assembly techniques in higher plants with large genomes.KeywordsSesamum inidum L.Sesame genomeSequencing platformsAssembly strategy