Serviksin invaziv skuamöz hücreli karsinomlarında HPV'nin PCR ve immünohistokimyasal yöntemlerle belirlenmesi

Abstract

Many epidemiological, clinicopaihological and molecular studies have shown the role of human papilloma virus (HPV) in the pathogenesis of the cervical carcinoma precursor lesions. Polimerase chain reaction (PCR), is very sensitive method for its establishment. Another method to identify HPV in cervival samples is immunohistochemistry (IHC). In this study, we aimed to determine HPV incidence in cervical invasive squamous cell carcinoma (ISCC) by using PCR and IHC and to investigate the correlation between HPV types and histological types. In our pathology department, 26 cases are investigated for HPV presence and types by PCR method. They are evaluated for HPV presence immunohistochemically by BSA-peroxidase (DAB) method. By PCR we found HPV 16 in 23%, 18 in 11.5%, 33 in 4%, 16+33 (mixt infection) in 4% of the cases. We didn't show HPV 6 and 11 in any of the cases. HPV positivity was 30.5%, immunohistochemically. In 5 of the 15 cases that were found to be negative by PCR, HPV was shown by IHC. The percentage of the cases proven to be HPV positive by PCR and/or IHC was 61.5%. When we compared the histological types with HPV types established by PCR, 18% of large cell keratinizing carcinoma (LCKC) was HPV 16 (+), 18% was 18 (+), 9% was 33 (+), 9% was 16+33 (+) and 46% was HPV(-); whereas 13% of large cell non-keratinizing carcinoma (LCNKC) was HPV 16(+), 18% was 18 (+), 69% was HPV(-) and 50% of small cell carcinoma was HPV 16(+), 50% was HPV(-). When we evaluated the histological type distribution oflHC-proven HPV positive cases, 37.5% was LCKC and 62.5% was LCNKC. We find the role and incidence of HPV in cervical carcinoma cases in our country comparable to global evidence; we conclude with the importance of screening programmes.