Abstract

Abstract. Aeromonas hydrophila strains isolated from diseased fish in South‐East Asia were studied for their virulence in naive tilapia, Oreochrommis aureus (Steindachner), and blue gourami, Trichogaster trichopterus (Pallas). Five of the most virulent strains used in this study shared a common resistance to the killing effect of naive fish serum. Other factors such as lack of autoagglutination in 0.2% acriflavine, instability after boiling, production of an S‐layer, and proteases and haemolysins did not correlate well with virulence. In addition, serotyping could not group all the virulent strains. Therefore, serum resistance is considered as a good indicator for screening virulence of A. hydrophila strains isolated from diseased fish in South‐East Asia.

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