Abstract

Background: β-hydroxybutyrate (BHB) can upregulate brain-derived neurotrophic factor (BDNF) in mice, but little is known about the associations between BHB and BDNF in humans. The primary aim here was to investigate whether ketosis (i.e., raised BHB levels), induced by a ketogenic supplement, influences serum levels of mature BDNF (mBDNF) and its precursor proBDNF in healthy older adults. A secondary aim was to determine the intra-individual stability (repeatability) of those biomarkers, measured as intra-class correlation coefficients (ICC).Method: Three of the arms in a 6-arm randomized cross-over trial were used for the current sub-study. Fifteen healthy volunteers, 65–75 y, 53% women, were tested once a week. Test oils, mixed in coffee and cream, were ingested after a 12-h fast. Labeled by their level of ketosis, the arms provided: sunflower oil (lowK); coconut oil (midK); caprylic acid + coconut oil (highK). Repeated blood samples were collected for 4 h after ingestion. Serum BDNF levels were analyzed for changes from baseline to 1, 2 and 4 h to compare the arms. Individual associations between BHB and BDNF were analyzed cross-sectionally and for a delayed response (changes in BHB 0–2 h to changes in BDNF at 0–4 h). ICC estimates were calculated from baseline levels from the three study days.Results: proBDNF increased more in highK vs. lowK between 0 and 4 h (z-score: β = 0.25, 95% CI 0.07–0.44; p = 0.007). Individual change in BHB 0–2 h, predicted change in proBDNF 0–4 h, (β = 0.40, CI 0.12–0.67; p = 0.006). Change in mBDNF was lower in highK vs. lowK at 0–2 h (β = −0.88, CI −1.37 to −0.40; p < 0.001) and cumulatively 0–4 h (β = −1.01, CI −1.75 to −0.27; p = 0.01), but this could not be predicted by BHB levels. ICC was 0.96 (95% CI 0.92–0.99) for proBDNF, and 0.72 (CI 0.47–0.89) for mBDNF.Conclusions: The findings support a link between changes in peripheral BHB and proBDNF in healthy older adults. For mBDNF, changes differed between arms but independent to BHB levels. Replication is warranted due to the small sample. Excellent repeatability encourages future investigations on proBDNF as a predictor of brain health.Clinical Trial Registration:ClinicalTrials.gov, NCT03904433.

Highlights

  • Ketogenic diets—which are based on strict carbohydrate restriction—have been used as a treatment for epilepsy since the 1920’s (Kossoff and Cervenka, 2020), and are increasingly investigated in other neurological conditions, including Alzheimer’s Disease (AD) (Stafstrom and Rho, 2012; Taylor et al, 2019)

  • Previous exploratory studies in human adults have found some associations between body β-hydroxybutyrate (BHB) and serum brain-derived neurotrophic factor (BDNF) within a ketogenic diet intervention (Mohorko et al, 2019), and between BHB and plasma BDNF in the context of exogenous ketone ingestion (Walsh et al, 2020)

  • After observing that age and body mass index (BMI) were higher and more skewed in their distribution among men compared to women, we concluded that it was not meaningful to conduct further stratified analyses since their interpretation would be too ambiguous in this small sample

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Summary

Introduction

Ketogenic diets—which are based on strict carbohydrate restriction—have been used as a treatment for epilepsy since the 1920’s (Kossoff and Cervenka, 2020), and are increasingly investigated in other neurological conditions, including Alzheimer’s Disease (AD) (Stafstrom and Rho, 2012; Taylor et al, 2019). Epigenetic brain signaling functions of BHB could be an additional mechanism underlying the observed effects, as such properties of BHB have been established during the past decade (Newman and Verdin, 2017). These signaling functions of BHB include upregulation of the expression of brain-derived neurotrophic factor (BDNF), as shown in mice (Marosi et al, 2016; Sleiman et al, 2016; Hu et al, 2018, 2020). Neither have associations between BHB and BDNF been reported in older adults

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