Abstract
BackgroundHepatocellular carcinoma (HCC) is one of the most common cancers in the world,and the identification of biomarkers for the early detection is a relevant target. The purpose of the study is to discover specific low molecular weight (LMW) serum peptidome biomarkers and establish a diagnostic pattern for HCC.MethodsWe undertook this pilot study using a combined application of magnetic beads with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique and ClinPro Tools v2.2 to detect 32 patients with HCC, 16 patients with chronic hepatitis (CH), 16 patients with liver cirrhosis (LC) and 16 healthy volunteers.ResultsThe results showed 49, 33 and 37 differential peptide peaks respectively appeared in HCC, LC and CH groups. A Supervised Neural Network (SNN) algorithm was used to set up the classification model. Eleven of the identified peaks at m/z 5247.62, 7637.05, 1450.87, 4054.21, 1073.37, 3883.64, 5064.37, 4644.96, 5805.51, 1866.47 and 6579.6 were used to construct the peptides patterns. According to the model, we could clearly distinguish between HCC patients and healthy controls as well as between LC or CH patients and healthy controls.ConclusionsThe study demonstrated that a combined application of magnetic beads with MALDI-TOF MB technique was suitable for identification of potential serum biomarkers for HCC and it is a promising way to establish a diagnostic pattern.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1503629821958720.
Highlights
Hepatocellular carcinoma (HCC) is one of the most common cancers in the world,and the identification of biomarkers for the early detection is a relevant target
Reagents and instruments The Autoflex III MALDI-TOF mass spectrometer, α-cyanohydroxy cinnamic acid (HCCA), Magnetic beads-based weak cation exchange chromatography (MB-WCX) kit and peptide calibration standard were purchased from Bruker Daltonics (Germany)
Serum low molecular weight (LMW) protein profile identification MALDI-TOF MS combined with Magnetic beads (MB)-WCX were used in the study to detect LMW protein profile spectra
Summary
Hepatocellular carcinoma (HCC) is one of the most common cancers in the world,and the identification of biomarkers for the early detection is a relevant target. The purpose of the study is to discover specific low molecular weight (LMW) serum peptidome biomarkers and establish a diagnostic pattern for HCC. HCC accounts for 90% of PLC [1]. HCC is the fifth most common human cancer, with approximately 750 000 new cases occurring worldwide every year [2]. HCC ranks the third in the annual global cancer mortality rates [3] and the average time from the discovery of symptoms to death is 6–20 months [1,4,5,6]. In China HCC is one of the leading causes of mortality and morbidity [7].
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