Serum PDGF-BB, sFlt-1 and U-II in patients with preeclampsia

Abstract

Objective To explore the clinical diagnostic value of joint detection of serum platelet-derived growth factor BB (PDGF-BB), soluble vascular endothelial growth factor receptor -1 (sFlt-1) and urotensinⅡ(U-Ⅱ) in preeclampsia disease. Methods The cases of obstetric patients suffering from preeclampsia in the Third People's Hospital of Liaocheng, Shandong Province between October 2012 and April 2014 were enrolled, including 96 cases of mild preeclampsia and 81 cases of severe preeclampsia.Totally 68 cases of normal pregnant women with similar age and gestational age were selected as control group. A case-control study was applied for the following investigations. The concentrations of serum PDGF-BB, sFlt-1 and U-Ⅱwere measured using ELISA. The diagnostic value of PDGF-BB, sFlt-1 and U-Ⅱalone or in combination for preeclampsia was analyzed and evaluated with receiver operating characteristic curve (ROC) and Logistic regression analysis. Results Serum concentrations of PDGF-BB, sFlt-1 and U-Ⅱin mild preeclampsia group were(80.45±21.87)ng/L, (23.03±6.67)μg/L and(4.54±1.02)ng/L, and those in severe preeclampsia group were(124.91±47.54)ng/L, (35.65±12.45)μg/L and(6.29±2.31)ng/L, while those in control group were(60.89±19.38)ng/L, (17.19±7.867)μg/L and(3.81±1.01)ng/L, respectively. The three parameters in mild preeclampsia group and severe preeclampsia group were significantly higher than those in control group (P<0.01; F value was 79.43, 79.28 and 50.72 respectively). In the same situation, these three indicators in severe preeclampsia group were significantly higher than those in mild group (P<0.01). Moreover, the AUC of serum PDGF-BB, sFlt-1 and U-Ⅱalone or in combination were 0.821, 0.786, 0.772 and 0.933, respectively. The differences between joint detection and three individual detections were statistically significant (P<0.05), as combined detection having a sensitivity of 95.7% and a specificity of 86.6%. Conclusion The combined detection of Serum PDGF-BB, sFlt-1 and U-Ⅱ had an important value in early assessment and treatment of preeclampsia.(Chin J Lab Med, 2015, 38: 528-531) Key words: Pre-eclampsia; Vascular endothelial growth factor receptor-1; Urotensins