Serum paraoxonase-1 activity and concentration are influenced by human immunodeficiency virus infection

Abstract

Background Higher high-density lipoprotein concentrations are associated with a better disease course in HIV-infected patients. Paraoxonase-1, an enzyme contained within high-density lipoproteins, is thought to hydrolyse oxidised lipids. The aim of the present study was to investigate the relationships between HIV infection and the circulating activity and concentration of paraoxonase-1, and the concentration of high-density lipoproteins, apolipoprotein A-I and oxidised low-density lipoproteins. Methods We studied patients with HIV infection ( n = 212) and healthy subjects ( n = 409). In all the participants we measured the relevant biochemical and genetic variables. The statistical associations between these variables and paraoxonase-1 activity and concentration were assessed using multiple linear regression analysis. Results Serum paraoxonase-1 activity was decreased ( P < 0.001) and its concentration was increased ( P = 0.017) in HIV-patients compared to the controls. HIV infected patients had lower HDL-cholesterol and apolipoprotein A-I concentrations. Multivariate regression analysis showed that serum paraoxonase-1 activity was associated with the CD4+ T lymphocyte count ( P < 0.05), apolipoprotein A-I ( P < 0.001), and paraoxonase-1 genetic polymorphisms ( P < 0.001). Paraoxonase-1 concentration was associated with that of serum β-2-microglobulin ( P < 0.001). Conclusions Both, paraoxonase-1 activity and concentration were influenced by HIV-infection and these were related to alterations in HDL composition and the immunological status of the patients.