Serum Paraoxonase 1 Activity Is Associated with Fatty Acid Composition of High Density Lipoprotein

Maryam Boshtam,Soheila Abdi,Mohsen Ani,Gholam Basati,Narges Jafari Dinani,Amirnader Emami Razavi,Marjan Mansourian,Morteza Pourfarzam,Seddigheh Asgary,Gholam Ali Naderi

doi:10.1155/2013/612035

Abstract

Introduction. Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). Fatty acid composition of HDL could affect its size and structure. On the other hand, PON1 activity is directly related to the structure of HDL. This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men. Methods. One hundred and forty healthy men participated in this research. HDL was separated by sequential ultracentrifugation, and its fatty acid composition was analyzed by gas chromatography. PON1 activity was measured spectrophotometrically using paraxon as substrate. Results. Serum PON1 activity was directly correlated with the amount of stearic acid and dihomo-gamma-linolenic acid (DGLA). PON1/HDL-C was directly correlated with the amount of miristic acid, stearic acid, and DGLA and was inversely correlated with total amount of ω6 fatty acids of HDL. Conclusion. The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. As dietary fats are the major determinants of serum lipids and lipoprotein composition, consuming some special dietary fatty acids may improve the activity of PON1 and thereby have beneficial effects on health.

Highlights

Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1)
Subjects were divided into three experimental groups in accordance with their serum HDL cholesterol (HDL-C) levels to analyze the association between serum PON1 activity and HDL fatty acid composition
There was no significant difference in monounsaturated fatty acids (MUFA) of HDL particles among three groups

Summary

Introduction

Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men. The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. Serum paraoxonase 1 (PON1) is a 45 kDa glycoprotein which can catalyze the hydrolysis of various organophosphates and nerve agents [1, 2] and metabolize some drugs and prodrugs by its lactonase activity [3]. This enzyme which is located on high density lipoprotein (HDL) particles protects low density lipoprotein (LDL) phospholipids against oxidation [4]. It has been indicated that replacement of dietary saturated fats with trans fats in healthy men and women leads to a small reduction in the serum PON1 activity [17]

Methods

Results

Discussion

Conclusion