Serum Organochlorine Pesticides Level CYP1A1 Genetic Polymorphism & Risk of Prostate Cancer Cases Control Study

Abstract

Background: Agriculture is the backbone of the economic and social development in the Sudan. 80% of the population depends for their living on agriculture. Organochlorines (OC's) were the first to be used against cotton pests, and control termites in sugarcane. In cancer CYP1A1 have dealt primarily with the metabolic activation of pro-carcinogens including polycyclic aromatic hydrocarbons and estradiol. Aim: The aim of this study is to determine the serum Organochlorine pesticides and PCBs (OC's) level, Cytochrome (CYP) 1A1 genetic polymorphisms and risk for Sudanese prostate cancer patients together with control groups, attending the Wad-Madani teaching hospital, Gezira State- Central Sudan. Method: 100 prostatic cancer patients and 100 controls (over 50 years old) will be studied. A questionnaire will be designed and Clinical examination will be performed including weight and heights. From venous blood (2.5ml) Serum will be separated and store at -80 ОC until analyses. The Organochlorine pesticides residues in serum will be measured by using a modified QuEChERS method by GC/MS. Soil, water and common food samples samples will be collected and storage until the analysis. For CYP1A1 genotyping examination, 2.5 ml of whole blood will be collected in 5 ml EDTA vacutainer tube. Buffy coat separate from plasma and store at -80 ОC until DNA extraction. DNA extraction for blood will be done by QIAamp DNA Mini Kits. PCR Technique will be used for laboratory genetic analysis by using Genotypes Restriction Enzymes Length Polymorphisms (RFLP). Estrogen (E1) level will be analyzed by Elisa technique with Elisa Kits. The study will help to examine one of the most important risk factors for prostate cancer in Sudan (Organochlorine & PCBs pesticides). Find the mutations for Cytochrome P-450 CYP (1A1) genotypes and the relationship between Estrogen (E1) levels and other risk factors.