Abstract

Background and Aim. It remains challenging to determine the inflammatory activity in Crohn's disease (CD) for lack of specific laboratory markers. Recent studies suggest that serum omentin-1 is associated with inflammatory response. We aimed to assess the potential of serum omentin-1 as a marker of disease activity in CD patients. Methods. Serum omentin-1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) in patients with CD (n = 240), functional gastrointestinal disorders (FGDs, n = 120), and healthy controls (HC, n = 60) and evaluated for correlation with disease activity. Expression of omentin-1 in colonic tissues from patients with CD was also analyzed by real-time PCR and Western blotting. Serum omentin-1 levels as an activity index were evaluated using a receiver operating characteristic (ROC) curve. Results. Serum omentin-1 concentrations were significantly decreased in active CD patients compared with patients in remission, FGDs, and HC (all P < 0.001). Expression of omentin-1 was decreased at mRNA and protein levels in inflamed colonic tissues in active CD than that in noninflamed colonic tissues. Serum omentin-1 levels were negatively correlated with disease activity in CD, better than C-reactive protein (CRP). Conclusion. Our results indicate that serum and colonic omentin-1 expressions are decreased in active CD patients. The correlation of serum omentin-1 with disease activity in CD is superior to that of CRP. Serum omentin-1 is a potential marker for CD disease activity.

Highlights

  • Genetic, infectious, and immunological factors may play roles in Crohn’s disease (CD), the etiology of CD remains idiopathic

  • Serum Omentin-1 Levels Are Decreased in Active CD Patients

  • Serum omentin-1 concentrations were significantly decreased in the active CD patients compared with control FGDs (201.29 ± 76.65 ng/mL versus 401.40 ± 108.36 ng/mL; P < 0.0001) (Figure 1)

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Summary

Introduction

Infectious, and immunological factors may play roles in Crohn’s disease (CD), the etiology of CD remains idiopathic. Crohn’s Disease Activity Index (CDAI) is still the most commonly used method to assess the inflammatory activity in CD [2]. It remains challenging to determine the inflammatory activity in Crohn’s disease (CD) for lack of specific laboratory markers. We aimed to assess the potential of serum omentin-1 as a marker of disease activity in CD patients. Serum omentin-1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) in patients with CD (n = 240), functional gastrointestinal disorders (FGDs, n = 120), and healthy controls (HC, n = 60) and evaluated for correlation with disease activity. Serum omentin-1 levels were negatively correlated with disease activity in CD, better than C-reactive protein (CRP). Our results indicate that serum and colonic omentin-1 expressions are decreased in active CD patients. Serum omentin-1 is a potential marker for CD disease activity

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