Serum-mediated adherence of feline granulocytes to microfilariae of Brugia pahangi in vitro: variations with parasite maturation.

Abstract

Feline eosinophils and neutrophils readily adhered in vitro to the sheaths of microfilariae of Brugia pahangi in the presence of suitable serum. Both cell types flattened along the surface of the parasite undergoing cytoplasmic changes which included degranulation. Adherence was dependent on properties of both the serum and the history of the microfilaria used. Two types of serum factor were found to mediate adherence. Heat labile factors were present in sera from infected and uninfected cats as well as in sera from other species. They were removed by preincubation of sera with zymosan suggesting that complement components were involved. This suggestion was supported by the demonstration of C3 on the surface of microfilariae participating in adherence reactions. A heat stable factor, present in the serum of less than 10% of infected cats, also mediated adherence. This factor was demonstrated to be IgG by immunoadsorption and immunofluorescence. The ability of the microfilariae to participate in the adherence reaction mediated by complement factor varied with maturation of the parasite. Microfilariae obtained directly from the uteri of adult worms, or produced in vitro, did not possess the ability to participate in adherence. Young blood microfilariae (i.e. taken from the blood of cats recently patent) were similar to the in vitro produced parasites; however, the majority of blood microfilariae from infections of greater than three weeks patency participated in this form of adherence. No difference between blood and uterine/in vitro microfilariae was seen in adherence reactions mediated by heat stable antibody.