Serum Long Non-Coding RNAs PVT1, HOTAIR, and NEAT1 as Potential Biomarkers in Egyptian Women with Breast Cancer.

Amal Ahmed Abd El-Fattah,Nancy Nabil Shahin,Amal Mohamed Kamal,Nermin Abdel Hamid Sadik,Olfat Gamil Shaker

doi:10.3390/biom11020301

Abstract

Long non-coding RNAs play an important role in tumor growth, angiogenesis, and metastasis in several types of cancer. However, the clinical significance of using lncRNAs as biomarkers for breast cancer diagnosis and prognosis is still poorly investigated. In this study, we analyzed the serum expression levels of lncRNAs PVT1, HOTAIR, NEAT1, and MALAT1, and their associated proteins, PAI-1, and OPN, in breast cancer patients compared to fibroadenoma patients and healthy subjects. Using quantitative real-time PCR (qRT-PCR), we compared the serum expression levels of the four circulating lncRNAs in patients with breast cancer (n = 50), fibroadenoma (n = 25), and healthy controls (n = 25). The serum levels of PAI-1 and OPN were measured using ELISA. Receiveroperating-characteristic (ROC) analysis and multivariate logistic regression were used to evaluate the diagnostic value of the selected parameters. The serum levels of HOTAIR, PAI-1, and OPN were significantly higher in breast cancer patients compared to controls and fibroadenoma patients. The serum level of PVT1 was significantly higher in breast cancer patients than in the controls, while that of NEAT1 was significantly lower in breast cancer patients compared to controls and fibroadenoma patients. Both ROC and multivariate logistic regression analyses revealed that PAI-1 has the greatest power in discriminating breast cancer from the control, whereas HOTAIR, PAI-1, and OPN have the greatest power in discriminating breast cancer from fibroadenoma patients. In conclusion, our data suggest that the serum levels of PVT1, HOTAIR, NEAT1, PAI-1, and OPN could serve as promising diagnostic biomarkers for breast cancer.

Highlights

Breast cancer is the second-ranked leading cause of cancer among women, impacting2.1 million women each year
The serum levels of HOX transcript antisense RNA (HOTAIR) and Plasmacytoma variant translocation 1 gene (PVT1) were significantly higher in the breast cancer patients than in the controls at p < 0.05, while the serum level of nuclear enriched abundant transcript 1 (NEAT1) was significantly lower in the breast cancer patients compared to the control group at p < 0.01 and no significant difference was observed in the serum level of MALATl in breast cancer patients
No significant difference was observed in the serum levels of PVT1 and MALAT1 of breast cancer patients compared to fibroadenoma

Summary

Introduction

Breast cancer is the second-ranked leading cause of cancer among women, impacting. It ranks fifth for causing cancer-associated deaths in women. In 2018, it was revealed that 627,000 women died from breast cancer, accounting for 15% of all cancer deaths among women [1]. While rates of breast cancer are higher among women in developed countries, rates are increasing in every region globally. Breast cancer can affect men [2]. In Egypt, according to the National Cancer

Methods

Results

Conclusion