Serum lipoprotein profile and oxidative stress biomarkers in Wistar rats fed drinking water containing iron and copper

Abstract

The aim of the research was to estimate the effect of different doses and combinations of iron and copper consumption with drinking water on lipid profile and oxidative stress biomarkers in albino Wistar rats serum. Rats were given drinking water containing 3 mg L �1 and 6 mg L �1 iron; copper 4.88 and 9.76 mg L �1 ; a mixture of 3 mg L �1 iron and 4.88 mg L �1 copper. Control group obtained pure drinking water. Total cholesterol, lipoprotein spectrum and markers of lipid and protein oxidation were analyzed. It has been seen that administration of iron in concentration of 6 mg L �1 induces lipid peroxidation and protein oxidation, while copper given in the maximal doses leads only to protein oxidation. Free radical oxidation in rats obtaining combination of iron and copper with drinking water was more expressed than in case of administration of single metals in the same doses. Consumption of maximal doses of isolated metals leads to more expressed atherogenic changes, while combination of both metals in lower doses did not affect serum lipoprotein significantly. The data obtained show that chemical interaction of iron and copper in the organism has an additive effect on some vital parameters in comparison to isolated metal administration.