Serum Level of Testosterone Predicts Disease Severity of Male COVID-19 Patients and is Related to T-Cell Immune Modulation by Transcriptome Analysis

Abstract

Background: Severe disease of COVID-19 and mortality occur more frequently in male patients than that in female patients may be related to higher level of testosterone. However, the diagnostic value of changes in the level of testosterone in predicting severe disease of male COVID-19 patients has not been determined yet.Methods: Sixty-one male COVID-19 patients admitted to the First Affiliated Hospital of Zhejiang University School of Medicine were enrolled. Serum samples at different stages of the patients after admission were collected and testosterone levels were detected to analyze the correlation between testosterone level and disease severity. Transcriptome analysis of PBMC was performed in 34 patients.Results: Testosterone levels at admission in male COVID-19 patients (4.7 nmol/L, IQR: 2.7~7.7 nmol/L) were significantly lower than those in healthy controls (19.5 nmol/L, IQR: 14.9~22.5). Testosterone levels in the non-ICU group increased gradually during the progression of the disease, while those in the ICU group remained low. In addition, testosterone level of enrolled patients in the second week after onset was significantly correlated with the severity of pneumonia, and ROC curve showed that testosterone level in the second week after onset was highly effective in predicting the severity of COVID-19. Transcriptome studies have found that testosterone levels of COVID-19 patients were associated with immune response, including T cell activation and regulation of lymphocyte activation. In addition, CD28 and Inositol Polyphosphate-4-Phosphatase Type II B (INPP4B) were found positively correlated with testosterone.Conclusions: Serum testosterone is an independent risk factor for predicting the severity of COVID-19 in male patients, and the level of serum testosterone in the second week after onset is valuable for evaluating the severity of COVID-19. Testosterone level is associated with T cell immune activation. The monitoring of serum testosterone should be highlighted in clinical treatment and the related mechanism should be further studied.Funding Information: This work was supported by the the National Natural Science Foundation of China (grant numbers 82072377 and 81971919). Declaration of Interests: All authors: No reported conflicts.Ethics Approval Statement: This study conformed to the ethical guidelines of the 1975 Declaration of Helsinki and was approved by the Institutional Review Board of the First Affiliated Hospital of Zhejiang University School of Medicine (IIT20210227A).