Abstract
The aim of this study was to investigate the effects of methylprednisolone treatment on serum leptin and ghrelin levels in healthy dogs ( n = 40). After 14 h of fasting, the dogs were injected intramuscularly with saline (control group) or methylprednisolone (1, 5 or 10 mg/kg). Blood samples were collected prior to (baseline) and 2, 3, 4, 8, 12 and 24 h subsequent to the treatments. Serum leptin and ghrelin were measured by radioimmunoassay. The mean baseline serum leptin and ghrelin were 2.5 ± 0.1 ng/mL ( n = 40) and 35.0 ± 2.1 pg/mL ( n = 40), respectively. In the control dogs, serum leptin, but not ghrelin levels showed a significant fluctuation during the 24 h observation period. Serum leptin increased significantly ( p < 0.05–0.01) between 2 and 12 h after 1 mg/kg of methylprednisolone. Serum leptin levels showed biphasic response to 5 mg/kg of methylprednisolone: its level decreased to 1.9 ± 0.1 ng/mL ( p < 0.01) at 2 h and increased at 12 h (2.6 ± 0.1 ng/mL) ( p < 0.01). In response to 10 mg/kg of methylprednisolone, serum leptin levels decreased significantly ( p < 0.01) for 24 h. Serum ghrelin levels decreased to 19 ± 5 pg/mL at 2–3 h ( p < 0.01) or increased to 87 ± 18 pg/mL at 3–8 h ( p < 0.05–0.01) after 1 mg/kg of methylprednisolone or 10 mg/kg of methylprednisolone, respectively. Serum ghrelin levels did not change at any time point during 24 h observation period after 5 mg/kg of methylprednisolone. There was a significant ( p < 0.001) inverse correlation ( r = −0.635) between serum leptin and ghrelin levels. In conclusion, we found that methylprednisolone increases or decreases serum leptin and ghrelin levels depending upon its dose and there is a negative correlation between serum leptin and ghrelin levels after methylprednisolone administration.
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