Abstract
Cryptosporidium spp. are responsible for moderate to severe diarrhea, mainly in children and immunocompromised patients. Using ELISA, the recognition of synthetic peptides generated from the sequences of the Cryptosporidium parvum gp40 and gp15 proteins by serum IgM and IgG antibodies from patients infected (cases) with Cryptosporidium hominis, C. parvum, and Cryptosporidium canis, and uninfected individuals (controls) was evaluated. A statistically significant difference (p = 0.0025) was found in terms of the recognition of peptides A133 and A32 between cases and controls. Additional studies are necessary to understand the potential of these peptides as vaccine candidates.
Highlights
Cryptosporidium spp. of the phylum Apicomplexa are protozoan parasites of medical and veterinary importance worldwide because they are causal agents of moderate to severe diarrhea, with higher prevalence in developing countries (Chalmers and Giles, 2010; Bouzid et al, 2013)
Of the 39 cases with cryptosporidiosis included in this study, C. parvum (19/39) was identified in 48.7%, C. hominis (13/39) in 33.3%, and C. canis in 2.6% (1/39) by analyzing the sequence of the SSU rRNA gene
In 7.7% of the cases (3/39), a coinfection of C. parvum and C. hominis was determined by qPCR, and in 2.6% of the cases (1/39), a coinfection of C. canis and C. hominis was determined (Table 1)
Summary
Cryptosporidium spp. of the phylum Apicomplexa are protozoan parasites of medical and veterinary importance worldwide because they are causal agents of moderate to severe diarrhea, with higher prevalence in developing countries (Chalmers and Giles, 2010; Bouzid et al, 2013). The Food and Drug Administration (FDA) has approved nitazoxanide as a drug against cryptosporidiosis with an effectiveness of 56%–96% in immunocompetent people (Checkley et al, 2015). Recent efforts to develop a vaccine against Cryptosporidium spp. have focused on the identification and characterization of immunogenic proteins (GP900, 27-kD, gp15/CP15, gp, CSL) involved in the adhesion and invasion of Cryptosporidium merozoites and sporozoites (Frost et al, 2005; Ajjampur et al, 2011; Allison et al, 2011; Avendaño et al, 2018). The objective of this study was to evaluate humoral immunity, as demonstrated in the recognition of synthetic peptides of the Cryptosporidium parvum gp and gp proteins by serum
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