Serum hormones during the estrous cycle and estrous behavior in heifers after administration of propylthiouracil and thyroxine

Abstract

Two experiments were conducted to assess the characteristics of thyroid-stimulating hormone (TSH) during the estrous cycle and to examine the effects of experimentally altered thyroid status on estrous behavior and hormonal concentrations in heifers. In Exp. 1, normal profiles of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and TSH were examined on days 2, 12, and 19 of the estrous cycle (day 0 = estrus) in six Holstein heifers. Baseline concentrations of LH were 1.5- to 2.1-fold higher on day 2 (P = .07) and day 19 (P < .01) than on day 12. Pulse frequency of LH was higher (P < .05) on days 12 and 19 than on day 2. For FSH, baseline concentrations were similar on all 3 d, but pulse frequency was higher (P < .05) on day 19 than on day 2 or day 12. Overall and baseline concentrations of TSH were similar on all 3 d. Pulse frequency of TSH was higher (P < .05) on day 12 than on day 19, but both were similar to that on day 2. In Exp. 2, secretion of TSH in Holstein heifers was altered by administering (i.v.; one-half of the daily dose given twice daily) either phosphate-buffered saline (SAL; n=6), propylthiouracil (PTU; 20 mg/100 kg body weight [BW]; n=6), or T 4 (1 mg/100 kg BW; n=5) from day 4 until the next estrus. Duration of estrous cycles during and after treatments, percentages of heifers exhibiting estrus, BW, and average daily gain were unaffected by treatment. Concentrations of TSH were higher (P < .05) in the afternoon than in the morning and higher (P = .06) in PTU heifers than in T 4 heifers. Treatment with T 4 during the treatment estrous cycle and for up to 6 d into the next estrous cycle increased (P < .01) concentrations of T 4 and T 3 compared to SAL and PTU. Treatment with PTU during the treatment cycle increased concentrations of T 4 (P < .05) and TSH (P = .06) compared to SAL. We conclude that TSH secretion was pulsatile, demonstrated a diurnal change, and varied inconsistently during the estrous cycle. Altering thyroid hormones and TSH secretion in heifers seemed to have no effect on estrous behavior or estrous cycles.