Abstract
The adipogenic transcription factor peroxisome proliferator-activated receptor-γ (PPARγ) plays a pivotal role in the regulation of whole body insulin sensitivity. Little, however, is known about hormonal and molecular modulation of PPARγ gene expression. Therefore, we investigated the temporal and conditional expression of PPARγ in a serum-free model of 3T3-L1 adipocyte differentiation. We show here that expression of PPARγ depends on the full set of known adipogenic stimuli and not on a single hormone/agent. Unexpectedly, an indeed marked but only transient peak of PPARγ expression (39±5-fold increase over basal on day 3 after hormonal stimulation) occurs during serum-free adipose conversion. To our knowledge, this finding is novel and probably remained hidden until now because of the common use of serum-containing preadipocyte culture systems. We conclude that maintenance, but not induction, of PPARγ gene expression in vitro must be achieved by one or more still unknown serum component(s).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
