Abstract
Yoder and Hofstad (2) found that certain Mycoplasma serotypes survived eight passages in serum-free broth whereas an unclassified isolate persisted in serum-free media. Mycoplasma gallisepticum, however, was found to require serum for growth. Their report recalls an experience with an unsatisfactory serum fraction lot in this laboratory that justifies report since it represents a potential hazard in research and diagnostic procedure. One particular lot of PPLO serum fraction (Difco) obtained from an intermediary dealer failed to support growth of stock cultures of M. gallisepticum. Meanwhile, 3 unidentified Mycoplasma isolates grew readily in broth medium containing the offending lot. These three isolates did not produce sinusitis, other signs of disease, or M. gallisepticum agglutinins in turkeys or chickens when inoculated by appropriate respiratory routes. Further, unlike M. gallisepticum, they did not agglutinate chicken red blood cells.
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