Serum Fluorescent Advanced Glycation End (F-AGE) products in gestational diabetes patients.

João Paulo Lobo Júnior,Marciane Welter,Geraldo Picheth,Dayane Alberton,Catiane Pompilio Brescansin,Fabiane Gomes De Moraes Rego,Izabella C R Santos-Weiss,Emanuel Maltempi De Souza

doi:10.1590/2359-3997000000238

João Paulo Lobo Júnior, Marciane Welter + Show 6 more

Open Access

https://doi.org/10.1590/2359-3997000000238

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Abstract

Advanced glycation end products (AGEs) are involved in the pathogenesis and complications of diabetes mellitus (DM). Gestational DM (GDM) is characterized by increased glycemia and oxidative stress, which are factors associated with high serum AGE concentrations. The aim of this study was to evaluate the utility of a serum fluorescence AGE (F-AGE) method as a screening tool for gestational diabetes. Serum samples from 225 GDM patients and 217 healthy pregnant women (healthy controls) were diluted 50-fold in phosphate-buffered saline, and the AGEs were estimated by fluorometric analysis (λEx 350 nm/ λEm 440 nm). No significant (P > 0.05) differences in AGE concentrations, expressed in Arbitrary Units (UA/mL × 104), were observed in the women with GDM or in the healthy controls. Furthermore, F-AGE concentrations did not change significantly during the pregnancy (12-32 weeks of gestation). Only the GDM group had a positive correlation (r = 0.421; P < 0.001) between F-AGEs and serum creatinine concentrations. It was not possible to distinguish women with gestational diabetes from the healthy controls on the basis of serum F-AGE concentrations.

Highlights

Advanced glycation end products (AGEs) are generated by the non-enzymatic reaction of a sugar ketone or aldehyde group with the free amino groups of proteins, amino acids, lipids, and nucleic acids under conditions of hyperglycemia and oxidative stress [1,2]
The fluorescence AGE (F-AGE) concentrations expressed as arbitrary units per unit volume (AU/mL) and per mass of protein (AU/g) were not statistically significant (P > 0.05) between the two groups (Table 1)
Serum AGEs can be detected by many analytical methods, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, radioreceptor assay, fluorescence spectroscopy, and High performance liquid chromatography (HPLC) [18,20,23,24]

Summary

Introduction

Advanced glycation end products (AGEs) are generated by the non-enzymatic reaction of a sugar ketone or aldehyde group with the free amino groups of proteins, amino acids, lipids, and nucleic acids under conditions of hyperglycemia and oxidative stress [1,2]. The AGE-RAGE interaction can lead to oxidative stress, production of growth factors and cytokines, chronic inflammatory responses, and cellular and vascular dysfunction [5,6]. Elevated AGEs concentrations are associated with several diseases, including diabetes mellitus (DM) [5,7,8]. DM is a pathology characterized by hyperglycemia, oxidative stress, inflammation, and the AGE-RAGE interaction is enhanced [1,5].

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