Serum enzyme activities in blood and milk in the different stage of lactation in holstein dairy cows

Radojica Djokovic,Zoran Ilic,Boban Jasovic,Vladimir Kurcubic,Marko Cincovic,Milos Petrovic,Miroslav Lalovic

doi:10.2298/bah1702193d

Abstract

The objective of this study was to determine correlation between serum blood and milk enzyme activities of aspartate-aminotransferase (AST), alanine- aminotransferase (ALT), alkaline-phosphatase (ALP in the 36 dairy Holstein cows divided into three groups according to production period. Group 1 consisted cows in the start of lactation (n = 12); Group 2 -consisted of early lactation cows (n=12) and Group 3 included mid lactation cows (n=15). Statistically significant higher (P<0.01) activity of AST in blood serum was established in early lactation groups of cows as compared to mid lactation group of cows. ALT activity showed a lower (P<0.01) serum activities in early lactation groups of cows than in the mid lactation cows. Higher values ALP in blood and milk are determined in early lactation groups of cows as compared to mid laltation cows, but without statistical significance (P>0.05). Research results showed possibility of mild degree of hepatic lesions, probably due to fat infiltration in early lactation cows. No significant difference (P>0.05) was observed in milk serum value for AST, ALT and ALP between the three groups of cows. No significant correlations among AST, ALT and ALP activities in blood and milk serum were determined (P>0.05) and shows that activity of these enzyme in the milk are not used as markers for early diagnosis of subclinical metabolic disease.

Highlights

The metabolic profile, a series of specific blood analytical tests, is routinely used to reveal metabolic problems in dairy cattle (Oetzel, 2004, Stengärde et al, 2008; Gross et al, 2011)
Diet in mid lactation consisted of 4.5 kg lucerne hay, 19 kg corn silage (30% Dry Matter, DM), 16 kg beet nodle silage, 9 kg concentrate (18% crude protein, Crude Protein (CP)) and 1.2 kg soybean expeller
The results of the serum blood and milk activities of AST, ALT, ALP in cows in the early and mid-lactation period and correlations among blood an milk serum enzyme activities are given in Tables 2 and 3

Summary

Introduction

The metabolic profile, a series of specific blood analytical tests, is routinely used to reveal metabolic problems in dairy cattle (Oetzel, 2004, Stengärde et al, 2008; Gross et al, 2011). Milk parameters originate from blood and food component and clarifying the appropriate relationships among these parameters individually in food blood and milk are useful in understanding the health and production status in animals (Jozwik et al.,2012; Liu et al, 2012; 2013). Blood serum ALT, AST, ALP and GGT activities were reported to be useful indicator of liver function for postpartum dairy cows (Bobe, 2004; Stojević et al, 2005). While little information is available concerning about the activity changes of ALT, AST, GGT and ALP in milk. The activities of these enzymes were monitored in milk and blood serum of cows and results of correlation analysis and regressive models showed a close relation between them (Liu et al, 2012, 2013; Ghadaa 2014). More practical attention has been given to detection of enzyme activity in milk and many enzymes have been proposed and listed a reliable markers for early diagnosis of subclinical disease (Babae et al, 2007; Katsoulos et al, 2010; Liu et al, 2012; 2013)

Objectives

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Results

Conclusion