Serum creatine kinase isoenzyme measurements in master male and female marathon runners

Abstract

This study investigated exercise‐induced muscle enzyme release in master runners by assessing the activity of total serum creatine kinase (total CK) and the concentration of the isoenzyme creatine kinase MB (CK‐MB). Subjects were 12 male (54 ± 9 years) and seven female (50 ± 6 years) master marathon runners. Premarathon (24 hours before) and postmarathon serum samples were collected (24, 48, 72, and 96 hours after the race). Total CK was measured enzymatically and CK‐MB concentration was assayed utilizing a double antibody procedure. The men's and women's 24 hour (1012 U/L, 782 U/L) and 48 hour (581 U/L, 441 U/L) postrace mean total CK activities were significantly (p≤0.05) elevated above prerace values (66 U/L, 119 U/L). CK‐MB concentration 24 hours postrace was also significantly (≤0.05) elevated from prerace values (0.27 ng/ml, 0.0 ng/ml) in both males (34.4 ng/ml) and females (16.7 ng/ml). The results of this study indicate that the pattern of exercise‐induced muscle enzyme release from the stress of ...