Serum concentrations of glucuronidated and sulfated bile acids in children with cholestasis

Abstract

Serum concentrations of nonglucuronidated-nonsulfated, glucuronidated, and sulfated bile acids in 9 control children and 16 children with cholestasis were quantitated by mass fragmentography. Total bile acid levels in control children were 19.55 ± 2.78 μmole/liter (mean ± SEM), and glucuronidated and sulfated bile acids comprised 2.6 ± 0.5 and 17 ± 3.1%, respectively. In 9 patients with congenital biliary atrasia, total bile acid levels were 167.34 ± 11.18 μmole/liter of which 2.1 ± 0.3% were glucuronidated and 15 ± 1.4% were sulfated. Lithocholic and 3β-hydroxy-5-cholenoic acids, which have hepatotoxic effects, were presented in only small amounts in cholestatic children, and they were almost all glucuronidated or sulfated. The percentages of glucuronidated bile acids in control and cholestatic children were lower than in healthy and cholestatic adults, which may be explained by the lower activity of UDP-glucuronyltransferase in neonatal liver.