Serum angiopoietin-like 3 levels are elevated in obese non diabetic men but are unaffected during an oral glucose tolerance test

Maria Fernanda Garcés,Diana Carolina Moreno-Ordóñez,Rubén Nogueiras,Jhoan Sebastian Roncancio-Muñoz,Carlos Diéguez,Ezequiel Lacunza,Julieth Daniela Buell-Acosta,Haiver Antonio Rodríguez-Navarro,Juan José Rincon-Ramírez,Elizabeth Sanchez,Alvaro Javier Burgos-Cardenas,Justo P Castaño,Roberto Franco-Vega,Estefania Pulido-Sánchez,Jorge Eduardo Caminos

doi:10.1038/s41598-020-77961-8

Abstract

This study aimed to determine ANGPTL3 serum levels in healthy young lean and obese non-diabetic men during an oral glucose tolerance test (OGTT) and correlate them with anthropometric, biochemical and hormonal parameters. A case–control study was carried out and 30 young obese non-diabetic (23.90 ± 3.84 years and BMI 37.92 ± 4.85 kg/m2) and 28 age-matched healthy lean (24.56 ± 3.50 years and BMI of 22.10 ± 1.72 kg/m2) men were included in this study. The primary outcome measures were serum basal ANGPTL3 and ANGPTL3–area under the curve (AUC) levels. The percentage of body fat was measured by dual-energy X-ray absorptiometry and biochemical, hormonal and insulin resistance indices were determined. Basal ANGPTL3 and ANGPTL3–AUC levels were significantly elevated (p < 0.05) in young obese subjects compared with lean subjects and were positively and significantly associated with different anthropometric measurements. Fasting ANGPTL3 serum levels were positively correlated with fasting insulin, leptin, Leptin/Adiponectin index and triglyceride—glucose index. Moreover, ANGPTL3–AUC was negatively correlated with Matsuda index. In this regard, chronically high ANGPTL3 levels in young obese subjects might favor triglyceride-rich lipoprotein clearance to replenish triglyceride stores by white adipose tissue rather than oxidative tissues.

Highlights

This study aimed to determine ANGPTL3 serum levels in healthy young lean and obese nondiabetic men during an oral glucose tolerance test (OGTT) and correlate them with anthropometric, biochemical and hormonal parameters
During the OGTT, serum glucose and insulin levels were significantly higher in the obese group compare with lean group at fasting, 30, 60, and 120 min (Figs. 1, 2 respectively, Table 2)
Glucose—Area Under the Curve (AUC) and insulin—AUC were significantly higher in obese compared with lean subjects (Figs. 1, 2, Table 2)

Summary

Introduction

This study aimed to determine ANGPTL3 serum levels in healthy young lean and obese nondiabetic men during an oral glucose tolerance test (OGTT) and correlate them with anthropometric, biochemical and hormonal parameters. Basal ANGPTL3 and ANGPTL3–AUC levels were significantly elevated (p < 0.05) in young obese subjects compared with lean subjects and were positively and significantly associated with different anthropometric measurements. ANGPTL3–AUC was negatively correlated with Matsuda index In this regard, chronically high ANGPTL3 levels in young obese subjects might favor triglyceride-rich lipoprotein clearance to replenish triglyceride stores by white adipose tissue rather than oxidative tissues. ANGPTL3 is a secreted glycoprotein mainly expressed in the liver and acts as a negative regulator of LPL and endothelial lipase activity in oxidative tissues such as brown adipose tissue (BAT) and skeletal m uscle[9]. Leptin-deficient ob/ob mice supplemented with recombinant leptin, showed lower gene expression and plasmatic levels of ANGPTL3 and normalization of plasmatic triglyceride levels[13]

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