Abstract
Background/Aims: Dendritic cells (DCs), antigen-presenting cells linking innate and adaptive immunity, are required for initiation of specific T cell-driven immune responses. Phosphoinositide-3-kinase (PI3K) suppresses proinflammatory cytokine production in DCs, which limits T helper (Th1) polarization. PI3K is in part effective by downregulation of transcription factor NF-κB. Downstream signaling elements of PI3K include serum- and glucocorticoid-inducible kinase 1 (SGK1) and its phosphorylation target N-myc downstream regulated gene 1 (NDRG1). The present study explored whether SGK1 and NDRG1 play a role in the regulation of NF-κB and DC-maturation. Methods: DCs were isolated from bone marrow (BMDCs) or spleen of mice lacking functional SGK1 (sgk1<sup>-/-</sup>) and corresponding wild type mice (sgk1<sup>+/+</sup>). Protein abundance was determined by Western blotting. Transcription was inhibited by siRNA. Abundance of maturation markers was quantified by flow cytometry. FITC-dextran uptake was determined to quantify phagocytosis. Results: NDRG1 was similarly expressed in sgk1<sup>+/+</sup> and sgk1<sup>-/-</sup>BMDCs, but SGK1-dependent phosphorylation of NDRG-1 was decreased in sgk1<sup>-/-</sup>BMDCs. Silencing of NDRG1 in sgk1<sup>+/+</sup>BMDCs as compared to control empty vector-treated BMDCs enhanced nuclear abundance of NF-κB subunit p65. Moreover, the abundance of phosphorylated NF-κB inhibitor IκBa, of phosphorylated IκB kinase (IKKa/ß) and of nuclear p65 were significantly higher in sgk1<sup>-/-</sup>BMDCs than in sgk1<sup>+/+</sup>BMDCs. Expression of maturation markers, MHC II, and CD86, was significantly larger and phagocytic capacity was significantly lower in sgk1<sup>-/-</sup> than in sgk1<sup>+/+</sup>BMDCs. Expression of CD86 and MHCII was also significantly higher in DCs isolated from the spleen of sgk1<sup>-/-</sup> mice than those from sgk1<sup>+/+</sup>mice. Conclusion: SGK1 and NDRG1 participate in the regulation of NF-κB signaling in and maturation of DCs.
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