Year-end Sale % OFF 
Abstract
This study was designed to identify serum and amniotic fluid (AF) metabolic profile changes in response to gestational diabetes mellitus (GDM) and explore the association with maternal–fetal outcomes. We established the GDM rat models by combining a high-fat diet (HFD) with an injection of low-dose streptozotocin (STZ), detected the fasting plasma glucose (FPG) of pregnant rats in the second and third trimester, and collected AF and fetal rats by cesarean section on gestational day 19 (GD19), as well as measuring the weight and crown–rump length (CRL) of fetal rats. We applied liquid chromatography–tandem mass spectrometry (LC-MS/MS) for the untargeted metabolomics analyses of serum and AF samples and then explored their correlation with maternal–fetal outcomes via the co-occurrence network. The results showed that 91 and 68 metabolites were upregulated and 125 and 78 metabolites were downregulated in serum and AF samples exposed to GDM, respectively. In maternal serum, the obvious alterations emerged in lipids and lipid-like molecules, while there were great changes in carbohydrate and carbohydrate conjugates, followed by amino acids, peptides, and analogs in amniotic fluid. The altered pathways both in serum and AF samples were amino acid, lipid, nucleotide, and vitamin metabolism pathways. In response to GDM, changes in the steroid hormone metabolic pathway occurred in serum, and an altered carbohydrate metabolism pathway was found in AF samples. Among differential metabolites in two kinds of samples, there were 34 common biochemicals shared by serum and AF samples, and a mutual significant association existed. These shared differential metabolites were implicated in several metabolism pathways, including choline, tryptophan, histidine, and nicotinate and nicotinamide metabolism, and among them, N1-methyl-4-pyridone-3-carboxamide, 5’-methylthioadenosine, and kynurenic acid were significantly associated with both maternal FPG and fetal growth. In conclusion, serum and AF metabolic profiles were remarkably altered in response to GDM. N1-Methyl-4-pyridone-3-carboxamide, 5’-methylthioadenosine, and kynurenic acid have the potential to be taken as biomarkers for maternal–fetal health status of GDM. The common and inter-related differential metabolites both in the serum and AF implied the feasibility of predicting fetal health outcomes via detecting the metabolites in maternal serum exposed to GDM.
Highlights
gestational diabetes mellitus (GDM) is defined as the varying degrees of impaired glucose tolerance occurring or first detected during pregnancy and is one of the most common metabolic disorders during pregnancy
The serum and amniotic fluid metabolite profiles were remarkably altered in response to GDM
In amniotic fluid (AF) samples exposed to GDM, we found the increased proportions of lipids and lipid-like molecules; carbohydrates and carbohydrate conjugates; and amino acids, peptides, and analogs, and we observed the elevated level of some metabolites implicated in amino acid, lipid, and carbohydrate metabolic pathways
Summary
GDM is defined as the varying degrees of impaired glucose tolerance occurring or first detected during pregnancy and is one of the most common metabolic disorders during pregnancy. GDM directly affects the intrauterine environment, inducing impaired function of the maternal–fetal unit and disturbed fetal glucose metabolism homeostasis and insulin secretion. Pregnant women with GDM typically exhibit increased insulin resistance and reduced insulin secretion, which bring about a succession of abnormal metabolisms that are embodied directly by the aberrant expression of corresponding metabolites. Detecting metabolite profiles of GDM and identifying potential disease-related metabolic pathways will promote the understanding of GDM pathogenesis and achieve effective prevention and treatment [9]. The fetus is directly exposed to AF, which is influenced by GDM [10], so identifying the differential AF metabolites and relevant metabolic pathways involved in the changed intrauterine environment will facilitate the understanding of the mechanism by which GDM has adverse effects on offspring
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
