Serum Amiloid-A'nın Sıçanlarda Formaldehit Kaynaklı Kupffer Hücre Apoptozundaki Rolü ve Astaksantin'in Bu Süreçteki Olası Koruyucu Etkileri

Abstract

The aim of this study is to investigate the alterations related to Serum amyloid-A in formaldehyde-induced apoptosis in Kupffer cells and to determine whether Astaxanthin has a protective effect against apoptosis. In this experiment, 32 rats were divided into 4 groups (n=8). The first group was named as control group, physiological saline was injected intraperitoneally to this group, and drinking water was given orally. In CH2O group, rats were injected with formaldehyde at a dose of 10 mg/kg daily intraperitoneally. The rats in CH2O+ATX16 and CH2O+ATX32 were injected with formaldehyde daily at a dose of 10 mg/kg intraperitoneally, and respectively 16 mg/kg and 32 mg/kg Astaxanthin were administered orally. Formaldehyde administration was caused by the highest and statistically significant Serum amyloid-A staining intensity (P<0.0125) and apoptotic index (P<0.05) in the CH2O group. Both doses of Astaxanthin administration reduced apoptosis in Kupffer cells but there were no significant differences in serum Serum amyloid-A levels between experimental groups (P>0.05). As a result, oral administration of Astaxanthin has been shown to reduce Serum Amyloid A, which increases due to exposure to formaldehyde, and possibly in this way, Kupffer cells successfully protect against formaldehyde-induced apoptosis. The subject should be examined more comprehensively