Abstract
BackgroundIt has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress. Oxidant-antioxidant imbalance is known to increase the conversion of deoxyguanosine to 8-hydroxy-2-deoxyguanosine (8-OHdG) in DNA. 8-OHdG can easily be measured using ELISA tests in serum or urine samples. In this study, we analysed serum 8-OHdG levels in horses with recurrent airway obstruction and in healthy controls.ResultsThe study material consisted of seven healthy horses and seven horses with symptomatic RAO. All horses were exposed to moldy hay and straw for 48 h to induce clinical exacerbation of RAO. The serum 8-OHdG levels were determined using the ELISA Highly Sensitive 8-OHdG kit. The difference between the levels of 8-OHdG in healthy and RAO-affected horses was significant. The median level of 8-OHdG was 0.044 ng/ml in the healthy controls versus 0.498 ng/ml in RAO horses (P = 0.0021).ConclusionsThe results of the study strongly suggest that DNA damage coexists in the course of equine RAO. We therefore propose that future research should aim at the development of new drugs that target pro-inflammatory molecules, since DNA damage appears to be the result of chronic inflammation.
Highlights
It has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress
Several studies have clearly shown that oxidative stress in the course of equine heaves results from an inflammatory response [3,4,5,6,7]
Grunwaldzki 47, 50‐366 Wrocław, Poland Full list of author information is available at the end of the article level of 8-OHdG has not been reported in the course of equine RAO
Summary
It has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress. Several studies have clearly shown that oxidative stress in the course of equine heaves results from an inflammatory response [3,4,5,6,7]. Grunwaldzki 47, 50‐366 Wrocław, Poland Full list of author information is available at the end of the article level of 8-OHdG has not been reported in the course of equine RAO. In this study, equine serum levels of 8-OHdG were determined.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
