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Abstract
The polypeptide composition of Sertoli cell enriched cultures (SCEC) and Sertoli cell only cultures (SCOC) obtained after germ cell removal was investigated. Cells were labelled with [ 35S]methionine and analyzed by one and/or two-dimensional gel electrophoresis. The one-dimensional electrophoretic analysis of SCEC and SCOC particulate fraction did not show any appreciable difference between the two profiles. The more detailed two-dimensional electrophoretic analysis showed the appearance in SCOC of three polypeptides undetectable in SCEC. The molecular weight of these molecules ranged between 44 and 48 kDa. The effect of FSH on the Sertoli cell membrane composition was also investigated. No qualitative differences were detected, although the hormone increased many molecular species including the polypeptides appearing in SCOC. On the basis of these results, the hypothesis that germ cells influence Sertoli cell metabolic parameters is discussed.
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