Abstract
In the presence of nanocatalyst, L-cysteine reduce HAuCl4 rapidly to form gold nanoparticles (AuNP), and a quick nanocatalytic preparation procedure was established for Au/AuNP sol with highly active surface enhanced Raman scattering (SERS) effect and good stability. The nanoreaction was also studied by absorption, resonance Rayleigh scattering (RRS), transmission electron microscopy (TEM) and energy spectra. In the selected conditions, the analyte heparin sodium (HS) could react with victoria blue 4 R (VB4r) to form associated complexes which have very weak SERS effect to make the SERS signals decrease. The SERS signals at 1617 cm−1 reduced linearly with HS concentration increasing. Upon addition of FeCl3, it hydrolyzed to form stable Fe(OH)3 sol platform that carried SERS active Au/AuNPs to enhance the sensitivity. Accordingly, we established a SERS quantitative analysis method in the sol substrate of Fe(OH)3-Au/AuNPs, with a linear range of 0.5–75 ng/mL HS and a detection limit of 0.2 ng/mL. HS in real samples was determined, with a relative standard deviation of 2.65–7.63% and a recovery of 99.3–101%.
Highlights
Substrate and the specific recognition of aptamer
A simple, rapid, sensitive and selective Surface-enhanced Raman scattering (SERS) quantitative analysis method was developed for detection of Heparin Sodium (HS) in the as-prepared AuNP sol substrate, using label-free molecular probe of Victoria blue 4 R (VB4r)
The results showed that the reaction was speeded up by the three nanocatalysts that enhance the redox electron transfer between CyS and AuCl4− to form more nucleuses to grow more small nanoparticles with high SERS activity
Summary
Substrate and the specific recognition of aptamer. Ye et al.[11] explained the nanogold catalysis in the synthesis of gold nanosol and the possibility of improving the SERS detection sensitivity. Wen et al.[12] reported a novel SERS method for trace human chorionic gonadotropin, based on the catalytic effect of nanosilver on the H2O2-HAuCl4 nanoreaction. There are no reports about the green and rapid preparation method for high SERS active gold nanosol, based on the AuNP catalysis of L-cysteine-HAuCl4 nanoreaction, and using the gold nanosol substrate to SERS quantitative analysis of trace analyte. SERS quantitative analysis of heparin sodium has not been reported. The L-cysteine-HAuCl4 nanoreaction was studied to prepare AuNP nanosol with high SERS activity. A simple, rapid, sensitive and selective SERS quantitative analysis method was developed for detection of HS in the as-prepared AuNP sol substrate, using label-free molecular probe of VB4r
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