Serratia sp. TKU016所生產蛋白酶及幾丁聚醣酶之純化與定性

Abstract

The chitosanase and protease producing strain, Serratia sp. TKU016, was isolated from the soil in Taiwan. The optimized condition for chitosanase and protease production were found when the culture was shaken at 30℃for 3 days in 100mL of medium contain 1% shrimp shell powder (SSP), 0.1 % K2HPO4 and 0.05 % MgSO4．7H2O (pH8). One chitosanase and one protease were purified by chromatography procedures of DEAE-Sepharose, and Sephacryl S-100. The molecular mass of the chitosanase and protease determined by SDS-PAGE was approximately 65 kDa and 53 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of chitosanase were pH 7、40℃、pH 4-8 and <80℃, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of protease were pH 11、40℃、pH 5-11 and <90℃,respectively. The chitosanase was inactivated by Mn2+. The protease showed good activity toward azocasein and azoalbumin as substrates, low activity with gelatin, fibrin, and elastin.