Serotypes and Multiresistant Salmonella sp. from Chicken Eggs and Laying Hens in Burkina Faso

Soutongnooma Caroline Bouda,Aïssata Wereme-N’Diaye,Assèta Kagambèga,Gilles Salvat,Sababenejo Alfred Traore,Nicolas Barro,Haoua Cisse,Hadiza Bawa Ibrahim,Laetitia Bonifait,Evariste Bako,Françoise Le Gall,Marianne Chemaly

doi:10.18483/ijsci.2231

Abstract

The study investigated the prevalence and antibioresistance profil of Salmonella sp. serovars isolated from eggs and poultry in stock farming. A total of 245 eggs and 98 laying hens fecal matters from different breeding sheds were collected. The samples were processed for identification and serotyping of Salmonella sp using microbiological standard methods and Kauffman-White scheme respectively. Salmonella sp isolates antibiotic susceptibility to antimicrobial agents was tested by disk diffusion method. A total of 63 Salmonella isolates were recovered with positive samples from eggs (11.8%) and from faecal matter (12.24%). The successful serotyping of 53/63 isolates revealed the presence of S. Typhimurium (11.11%), S. Kentucky (1.59%), S. Ouakam (1.59%), S. Brancaster (6.35%), S Hato (6.35%), S. Essen (3.17%), S. Cannstatt (1.59%), and S. Derby (36.51%). Ten strains (15.87%) were untypable and ten (15.87%) belong to different serogroups such F and O. All the serotypes shown resistance to at least one antibiotic while, 41 (65.08%) were multi-resistant to Erythromycin, Streptomycin, Tetracycline, Ceftriaxon, while high sensitivity was recorded for Chloramphenicol, Ciprofloxacin, Nalidixic acid, Imipenem, Cephalexin, Sulfamethoxazole–trimethoprim and Colistin Sulfate. These results suggest that eggs from stock farming are contaminated and harbour resistant Salmonella sp. It highlights worry in antibiotics use in stock farming, the need for farm workers and consumers education about safe handling of eggs.

Highlights

Salmonella is one of the major causes of foodborne disease outbreaks (Naik et al, 2015; Feasey et al, 2012; Sharkawy et al, 2017)
Chicken and eggs in particular continue to be identified as important sources for human Salmonellosis (Van Schothorst and Notermans, 1980; Tauxe, 1996; Thong et al, 2002, Finstad et al, 2010; Mead et al, 2010)
Out of the positive samples a total of 63 Salmonella isolates were obtained from eggshell (31 isolates), from yolk and albumen (13 isolates) and from over shoes (19 isolates) and serotyped (Tables 1, 2)

Summary

Introduction

Salmonella is one of the major causes of foodborne disease outbreaks (Naik et al, 2015; Feasey et al, 2012; Sharkawy et al, 2017). Among the most important microorganisms, Salmonella spp. may be considered one of the most circulating and frequent foodborne agents in the world (CDC, 2016; EFSA, 2017) and may cause significant damage to the poultry industry as well as to public health. Products of avian origin represent 47% of salmonellosis sources in humans (CDC, 2016). In poultry products Salmonella spp. was the main cause of early warnings in the developed countries these last years (RASFF, 2018). Salmonella spp control is a major concern for poultry producers in several countries (Fonseca et al, 2019). In developing country the scarce data are available on the role of poultry and product in Salmonella sp epidemiology because of the lack of epidemiological surveillance systems

Methods

Results

Discussion

Conclusion