Year-end Sale % OFF 
Abstract
BackgroundSalmonella has been considered as one of the most important foodborne pathogens that threatened breeding industry and public health. To investigate the prevalence and characterization of Salmonella isolated from duck farms and a slaughterhouse in Shandong province, a total of 49 Salmonella strains were isolated from 2342 samples from four duck farms and one duck slaughterhouse in Jinan and Tai’an, Shandong province, China.ResultsAmong the isolates, S. Enteritidis (20/49, 40.8%) and S. Anatum (10/49, 20.4%) were the most prevalent, and high resistance rates were detected for erythromycin (49/49, 100.0%) and nalidixic acid (47/49, 95.9%). Class I integrons were detected in 17 isolates (34.7%17/49), which contained gene cassettes aadA7 + aac3-Id(15/17) and aadA5 + dfrA17 (2/17). Eleven different kinds of resistance genes were detected while blaTEM(36/49, 73.5%) was the most prevalent, followed by sul2(14/49, 28.6%). Thirteen virulence genes were tested, and all of the strains carried invA, hilA and sipA. Multilocus sequence typing (MLST) results showed that seven sequence types (STs) were identified; ST11 was the most prevalent ST (20/49, 40.8%), followed by ST2441 (10/49, 20.4%). There was a strong correlation between STs and serovars. The results of pulsed field gel electrophoresis(PFGE) showed that 39 PFGE patterns were generated from 49 Salmonella strains. PFGE patterns were mostly diverse and revealed high similarity between the isolates from the same sampling sites.ConclusionsThe presence of Salmonella infections among duck farms revealed that ducks could also be potential reservoirs for Salmonella. The high resistance rates against commonly used antimicrobials suggested a need for more reasonable use of antimicrobials, as well as for investigating substitutes for antimicrobials.
Highlights
Salmonella has been considered as one of the most important foodborne pathogens that threatened breeding industry and public health
Isolation and serotyping of Salmonella A total of 49 Salmonella strains were isolated from 2342 samples obtained from four large-scaleduck farms and one slaughterhouse, having an isolation rate of 2.1%
The prevalence of positive samples was 15.3, 1.9, 1.4, 4.0 and 0.3% in farms 1, 2, 3 and 4 and in the slaughterhouse, respectfully (Table 3).The positive rate for Salmonella in duck livers from the diseased duck farms (12.3%, 10/81) was higher than that in duck livers from the slaughterhouse (1.2%, 1/83).In addition, a Salmonella strain was isolated from duck feed
Summary
Salmonella has been considered as one of the most important foodborne pathogens that threatened breeding industry and public health. Salmonella has been considered to be one of the most important foodborne pathogens associated with public health worldwide, and has been frequently reported in recent years [1, 2], including topics such as its route through the food chain to humans. Yang et al BMC Microbiology (2019) 19:202 widespread utilization of antibiotics, antimicrobial-resistant and even multidrug-resistant Salmonella strains have emerged and spread worldwide, and have seriously threatened global public health [8,9,10]. PFGE is used by laboratories around the world for its high resolution and repeatability, and is widely considered to be the gold standard for molecular typing.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
