Serotype and serum sensitivity of Pseudomonas aeruginosa from children with cystic fibrosis: Longitudinal studies and typing with monoclonal antibodies

Abstract

Pseudomonas aeruginosa isolated from the respiratory tract of children with cystic fibrosis from Perth, Sydney or Brisbane, were serotyped with polyclonal antisera using the International Antigenic Serotyping System. Predominant strains were untypable among isolates from Brisbane (86% of 50 strains) and Sydney (60% of 50 strains) but 64% of the 408 isolates from Perth were polyagglutinating. The frequency distributions of typable strains showed differences from those reported in clinics in the northern hemisphere, but it appears that these local differences do not affect the emergence of strains with defective lipopolysaccharide antigens. Isolates from patients studied longitudinally showed correlation between duration of colonization, appearance of untypable or polyagglutinating strains and sensitivity to normal serum. Mucoid colonies were found more commonly from patients colonized for at least 12 months but, overall, there was no significant association between serotype and mucoid colonial morphology. Commercial monoclonal antibodies did not react with polyagglutinating or untypable strains and gave identical results to the polyvalent typing system with strains classified as typable.