Serotonin systems in three socially communicating teleost species, the grunting toadfish (Allenbatrachus grunniens), a South American marine catfish (Ariopsis seemanni), and the upside-down catfish (Synodontis nigriventris)

Abstract

We investigated immunohistochemically the distribution of serotonergic cell populations in three teleost species (one toadfish, Allenbatrachus grunniens, and two catfishes, Synodontis nigriventris and Ariopsis seemanni). All three species exhibited large populations of 5-HT positive neurons in the paraventricular organ (PVO) and the dorsal (Hd) and caudal (Hc) periventricular hypothalamic zones, plus a smaller one in the periventricular pretectum, a few cells in the pineal stalk, and – only in catfishes – in the preoptic region. Furthermore, the rhombencephalic superior and inferior raphe always contained ample serotonergic cells. In each species, a neuronal mass extended into the hypothalamic lateral recess. Only in the toadfish, did this intraventricular structure contain serotonergic cells and arise from Hd, whereas in the catfishes it emerged from medially and represents the dorsal tuberal nucleus seen in other catfishes as well. Serotonergic cells in PVO, Hd and Hc were liquor-contacting. Those of the PVO extended into the midline area of the periventricular posterior tubercular nucleus in both catfishes. Dopaminergic, liquor-contacting neurons were additionally investigated using an antibody against tyrosine hydroxylase (TH) in S. nigriventris showing that TH was never co-localized with serotonin. Because TH antibodies are known to reveal mostly or only the TH1 enzyme, we hypothesize that th1-expressing dopamine cells (unlike th2-expressing ones) do not co-localize with serotonin. Since the three investigated species engage in social communication using swim bladder associated musculature, we investigated the serotonergic innervation of the hindbrain vocal or electromotor nuclei initiating the social signal. We found in all three species serotonergic fibers seemingly originating from close-by serotonergic neurons of inferior raphe or anterior spinal cord. Minor differences appear to be rather species-specific than dependent on the type of social communication.