Abstract
The effects of serotonin on ion channel activity in epithelial cells from rat choroid plexus were examined. Serotonin (50 nM, 500 nM and 1 μM) stimulated channel activity in cell-attached patches. The current-voltage (I–V) relationship for the serotonin-activated channel gave a conductance of 26.6 ± 1.5 pS and the current reserved at an applied electrode potential (−V P) = 15.3 ± 3.3 mV with a KCl-rich electrode solution (n= 8). Similar I–V relationships were obtained using electrode solutions in which K + was replaced by other cations (Na + and N-methyl-D-glucamine), suggesting that the serotonin-activated channels are selective to Cl −. The effect of 1 μM serotonin on channel activity was inhibited by ritanserin (30 and 100 nM) which has a high affinity for serotonin 5-HT 1C receptors and 5-HT 2 receptors. Spiperone (30 nM), which binds weakly to 5-HT 1C receptors but has a high affinity for 5-HT 2 receptors, did not inhibit the actions of serotonin. These data suggest that serotonin increases Cl − channel activity by acting on the 5-HT 1C receptors on the epithelium.
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