Seroprevalence of West Nile fever virus in horses in the Belgrade epizootiological area

Ljubisa Veljovic,Jadranka Zutic,Slobodan Stanojevic,Branislav Kureljusic,Vesna Milicevic,Nemanja Jezdimirovic,Ivan Pavlovic,Jelena Maksimovic-Zoric,Vladimir Radosavljevic

doi:10.2298/vetgl200207011v

Ljubisa Veljovic, Jadranka Zutic + Show 7 more

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https://doi.org/10.2298/vetgl200207011v

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Abstract

West Nile fever is a vector borne viral disease that can affect humans, horses, birds and sometimes other species of animals. Every year West Nile fever is detected in the human population in Serbia. The disease often occurs in a subclinical form, but most clinically evident cases occur in horses. Therefore, horses are recommended as a sentinel species for monitoring the general incidence of West Nile fever in a specific territory. Our goal was to determine the prevalence of antibodies against West Nile fever virus in horses in the Belgrade epizootiological area. We examined serum samples from 77 horses to determine the seroprevalence of West Nile fever virus in horses throughout the city of Belgrade. Sera were tested by commercial ELISA tests for detection of specific IgG-class antibodies to West Nile fever virus and for the detection of specific IgM-class antibodies to confirm the presence of old and acute (recent) infections in horses. The results confirmed that West Nile fever virus is widespread, detected in 70.1% of the surveyed horse population in Belgrade, and we also detected 5.1% of acute cases had occurred due to horses being infected in 2019. The seroprevalence of West Nile virus in the horse population in the municipality of Belgrade is increasing.

Highlights

West Nile fever is a vector-borne zoonosis of viral aetiology that can affect horses, birds and sometimes other species of animals besides humans (Stelle et al, 2000)
Out of 77 horse sera examined by the ELISA test, 54 sera (70.1%) were positive, while 23 sera (29.9%) were negative for the presence of specific IgG-class antibodies against West Nile virus (Table 1)
From 77 horse sera examined by the ELISA test for the presence of West Nile fever specific IgM-class antibodies, 4 sera (5.1%) were IgM positive, while 73 horse sera (94.9%) were negative

Summary

Introduction

West Nile fever is a vector-borne zoonosis of viral aetiology that can affect horses, birds and sometimes other species of animals besides humans (Stelle et al, 2000). Antibodies against West Nile fever virus have been detected sporadically in sera of other animal species such as dogs, cats, bats, squirrels, deer, sheep, alpacas, llamas, camels and more (Durand et al, 2012; OIE Ter. Manual, 2019). The first West Nile virus strain was isolated from a human patient in Uganda in 1937 (Bakonyi et al, 2006). The first reported outbreak of West Nile virus infection in humans in Serbia was detected in 2012 (Popović et al, 2013). No antivirals or other drugs are known to be effective in the prevention or treatment of West Nile virus infection (Campbell et al 2002)

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