Abstract
We identified a new polyomavirus in skin lesions from a patient with trichodysplasia spinulosa (TS). Apart from TS being an extremely rare disease, little is known of its epidemiology. On the basis of knowledge regarding other polyomaviruses, we anticipated that infections with trichodysplasia spinulosa–associated polyomavirus (TSV) occur frequently and become symptomatic only in immunocompromised patients. To investigate this hypothesis, we developed and used a Luminex-based TSV viral protein 1 immunoassay, excluded cross-reactivity with phylogenetically related Merkel cell polyomavirus, and measured TSV seroreactivity. Highest reactivity was found in a TS patient. In 528 healthy persons in the Netherlands, a wide range of seroreactivities was measured and resulted in an overall TSV seroprevalence of 70% (range 10% in small children to 80% in adults). In 80 renal transplant patients, seroprevalence was 89%. Infection with the new TSV polyomavirus is common and occurs primarily at a young age.
Highlights
We identified a new polyomavirus in skin lesions from a patient with trichodysplasia spinulosa (TS)
In plucked spicules of a TS patient, we recently identified a new human polyomavirus virus known as TS-associated polyomavirus (TSV) [9]
Development of the trichodysplasia spinulosa–associated polyomavirus (TSV) viral protein 1 (VP1) Immunoassay To measure seroreactivity against TSV, an immunoassay was developed with TSV VP1 antigen expressed as a GST-fusion protein in E. coli
Summary
We identified a new polyomavirus in skin lesions from a patient with trichodysplasia spinulosa (TS). On the basis of knowledge regarding other polyomaviruses, we anticipated that infections with trichodysplasia spinulosa–associated polyomavirus (TSV) occur frequently and become symptomatic only in immunocompromised patients. To investigate this hypothesis, we developed and used a Luminex-based TSV viral protein 1 immunoassay, excluded cross-reactivity with phylogenetically related Merkel cell polyomavirus, and measured TSV seroreactivity. In plucked spicules of a TS patient, we recently identified a new human polyomavirus virus known as TS-associated polyomavirus (TSV) [9] This finding has been recently confirmed by Matthews et al [12]. Seroepidemiologic data for BKV, JCV, and MCPyV indicate that human polyomavirus infections are ubiquitous and generally occur without apparent disease. We show that TSV is a common infection in the general population and in immunocompromised patients, and discuss the relevance of our findings with respect to TSV-induced disease
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
